An rxr/usp homolog from the parasitic nematode, Dirofilaria immitis.
Identifieur interne : 008865 ( Main/Exploration ); précédent : 008864; suivant : 008866An rxr/usp homolog from the parasitic nematode, Dirofilaria immitis.
Auteurs : Cathy Shea [États-Unis] ; David Hough ; Jianping Xiao ; George Tzertzinis ; Claude V. MainaSource :
- Gene [ 0378-1119 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN complémentaire (), ADN complémentaire (génétique), ADN des helminthes (), ADN des helminthes (génétique), Activation de la transcription, Alignement de séquences, Analyse de séquence d'ADN, Animaux, Dirofilaria immitis (croissance et développement), Dirofilaria immitis (génétique), Données de séquences moléculaires, Facteurs de transcription (génétique), Femelle, Lignée cellulaire, Mâle, Phylogénie, Protéines nucléaires (génétique), Récepteurs X des rétinoïdes, Récepteurs à l'acide rétinoïque (génétique), Régulation de l'expression des gènes au cours du développement, Similitude de séquences d'acides aminés, Sites de fixation (génétique), Séquence d'acides aminés.
- MESH :
- croissance et développement : Dirofilaria immitis.
- génétique : ADN complémentaire, ADN des helminthes, Dirofilaria immitis, Facteurs de transcription, Protéines nucléaires, Récepteurs à l'acide rétinoïque, Sites de fixation.
- ADN complémentaire, ADN des helminthes, Activation de la transcription, Alignement de séquences, Analyse de séquence d'ADN, Animaux, Données de séquences moléculaires, Femelle, Lignée cellulaire, Mâle, Phylogénie, Récepteurs X des rétinoïdes, Régulation de l'expression des gènes au cours du développement, Similitude de séquences d'acides aminés, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Binding Sites (genetics), Cell Line, DNA, Complementary (chemistry), DNA, Complementary (genetics), DNA, Helminth (chemistry), DNA, Helminth (genetics), Dirofilaria immitis (genetics), Dirofilaria immitis (growth & development), Female, Gene Expression Regulation, Developmental, Male, Molecular Sequence Data, Nuclear Proteins (genetics), Phylogeny, Receptors, Retinoic Acid (genetics), Retinoid X Receptors, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcription Factors (genetics), Transcriptional Activation.
- MESH :
- chemical , chemistry : DNA, Complementary, DNA, Helminth.
- genetics : Binding Sites, DNA, Complementary, DNA, Helminth, Dirofilaria immitis, Nuclear Proteins, Receptors, Retinoic Acid, Transcription Factors.
- growth & development : Dirofilaria immitis.
- Amino Acid Sequence, Animals, Cell Line, Female, Gene Expression Regulation, Developmental, Male, Molecular Sequence Data, Phylogeny, Retinoid X Receptors, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcriptional Activation.
Abstract
Filarial parasites are responsible for several serious human diseases with symptoms such as lymphoedema, elephantiasis, and blindness. An understanding of how these parasites pass through developmental checkpoints may suggest potential targets for intervention. A useful model system for the study of the human parasites is the closely related nematode Dirofilaria immitis, the causative agent of dog heartworm disease. In D. immitis, molting from the third to the fourth larval stage can be induced in vitro by the insect molting hormone, 20-hydroxyecdysone, suggesting that this, or some related steroid, may play a similar role in the development of D. immitis. The holoreceptor of 20-hydroxyecdysone consists of two nuclear receptors (NRs) ecdysone receptor (EcR) and ultraspiracle (USP), USP being the insect orthologue of the vertebrate RXR. We have identified a D. immitis rxr/usp, Di-rxr-1 (NR2B4). Di-RXR-1 can bind in vitro to EcR and DHR38, both known insect USP partners. Like, USP, it activates transcription in Drosophila Schneider S2 cells in a 20-hydroxyecdysone-dependent manner, via its interaction with the endogenous EcR protein. By Northern blot analysis, Di-rxr-1 mRNA is detected in adult females, but not in males. This is the first characterization of a nematode rxr/usp.
PubMed: 14693382
Affiliations:
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Le document en format XML
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Maina</name></author></analytic><series><title level="j">Gene</title><idno type="ISSN">0378-1119</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Binding Sites (genetics)</term><term>Cell Line</term><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (genetics)</term><term>DNA, Helminth (chemistry)</term><term>DNA, Helminth (genetics)</term><term>Dirofilaria immitis (genetics)</term><term>Dirofilaria immitis (growth & development)</term><term>Female</term><term>Gene Expression Regulation, Developmental</term><term>Male</term><term>Molecular Sequence Data</term><term>Nuclear Proteins (genetics)</term><term>Phylogeny</term><term>Receptors, Retinoic Acid (genetics)</term><term>Retinoid X Receptors</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Amino Acid</term><term>Transcription Factors (genetics)</term><term>Transcriptional Activation</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire ()</term><term>ADN complémentaire (génétique)</term><term>ADN des helminthes ()</term><term>ADN des helminthes (génétique)</term><term>Activation de la transcription</term><term>Alignement de séquences</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Dirofilaria immitis (croissance et développement)</term><term>Dirofilaria immitis (génétique)</term><term>Données de séquences moléculaires</term><term>Facteurs de transcription (génétique)</term><term>Femelle</term><term>Lignée cellulaire</term><term>Mâle</term><term>Phylogénie</term><term>Protéines nucléaires (génétique)</term><term>Récepteurs X des rétinoïdes</term><term>Récepteurs à l'acide rétinoïque (génétique)</term><term>Régulation de l'expression des gènes au cours du développement</term><term>Similitude de séquences d'acides aminés</term><term>Sites de fixation (génétique)</term><term>Séquence d'acides aminés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term><term>DNA, Helminth</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Dirofilaria immitis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Binding Sites</term><term>DNA, Complementary</term><term>DNA, Helminth</term><term>Dirofilaria immitis</term><term>Nuclear Proteins</term><term>Receptors, Retinoic Acid</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Dirofilaria immitis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ADN des helminthes</term><term>Dirofilaria immitis</term><term>Facteurs de transcription</term><term>Protéines nucléaires</term><term>Récepteurs à l'acide rétinoïque</term><term>Sites de fixation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Line</term><term>Female</term><term>Gene Expression Regulation, Developmental</term><term>Male</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Retinoid X Receptors</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Amino Acid</term><term>Transcriptional Activation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>ADN des helminthes</term><term>Activation de la transcription</term><term>Alignement de séquences</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Lignée cellulaire</term><term>Mâle</term><term>Phylogénie</term><term>Récepteurs X des rétinoïdes</term><term>Régulation de l'expression des gènes au cours du développement</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Filarial parasites are responsible for several serious human diseases with symptoms such as lymphoedema, elephantiasis, and blindness. An understanding of how these parasites pass through developmental checkpoints may suggest potential targets for intervention. A useful model system for the study of the human parasites is the closely related nematode Dirofilaria immitis, the causative agent of dog heartworm disease. In D. immitis, molting from the third to the fourth larval stage can be induced in vitro by the insect molting hormone, 20-hydroxyecdysone, suggesting that this, or some related steroid, may play a similar role in the development of D. immitis. The holoreceptor of 20-hydroxyecdysone consists of two nuclear receptors (NRs) ecdysone receptor (EcR) and ultraspiracle (USP), USP being the insect orthologue of the vertebrate RXR. We have identified a D. immitis rxr/usp, Di-rxr-1 (NR2B4). Di-RXR-1 can bind in vitro to EcR and DHR38, both known insect USP partners. Like, USP, it activates transcription in Drosophila Schneider S2 cells in a 20-hydroxyecdysone-dependent manner, via its interaction with the endogenous EcR protein. By Northern blot analysis, Di-rxr-1 mRNA is detected in adult females, but not in males. This is the first characterization of a nematode rxr/usp.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Hough, David" sort="Hough, David" uniqKey="Hough D" first="David" last="Hough">David Hough</name><name sortKey="Maina, Claude V" sort="Maina, Claude V" uniqKey="Maina C" first="Claude V" last="Maina">Claude V. Maina</name><name sortKey="Tzertzinis, George" sort="Tzertzinis, George" uniqKey="Tzertzinis G" first="George" last="Tzertzinis">George Tzertzinis</name><name sortKey="Xiao, Jianping" sort="Xiao, Jianping" uniqKey="Xiao J" first="Jianping" last="Xiao">Jianping Xiao</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Shea, Cathy" sort="Shea, Cathy" uniqKey="Shea C" first="Cathy" last="Shea">Cathy Shea</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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